Construction of human antisense BMP-2 retrovirus expression vectors and its biological effects on human osteosarcoma cells
- VernacularTitle:反义人骨形态发生蛋白2基因逆转录病毒表达载体的构建及其对骨肉瘤细胞生物学行为的影响
- Author:
Wen YUE
;
Lianjia YANG
;
Wei YAN
- Publication Type:Journal Article
- Keywords:
Bone morphogenetic proteins;
Osteosarcoma;
DNA,antisense;
Mutagenesis,insertional;
Genetic vectors
- From:
Chinese Journal of Orthopaedics
1996;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe into the mechanisms of bone morphogenetic protein-2 (BMP-2) in osteosarcoma and to provide basis for gene therapy. Methods A 1.0 kb cDNA fragment of human BMP-2 gene was inserted reversely into PDOR and Human antisense BMP-2 retrovirus expression vector was constructed. The recombinant retroviral vector was transfected into human osteosarcoma cells OS-9901 with liposome AMINE that expressed abundant BMP. Positive cell clones were selected with G 418. The expression of BMP and proliferating cell nuclear antigen (PCNA) was determined by immunohistochemical ABC methods. The osteosarcoma cell cycle was analysed by flowcytometry, and the changes were observed by electronmicroscope. Results The expression of cellular BMP and PCNA in the transfected osteosarcoma cells decreased obviously (t=24.01， 26.09, respectively, P
