Influence of vagus nerve stimulation upon glutamate receptor and GABA_A receptor in nucleus tract solitary of the kainate induced epileptic rat
- VernacularTitle:迷走神经刺激对红藻氨酸致癫痫大鼠孤束核内谷氨酸受体和?-氨基丁酸受体的影响
- Author:
Xin WANG
;
Yan ZHANG
- Publication Type:Journal Article
- Keywords:
Epilepsy;
? Receptors, glutamate;
? Receptor, GABA A;
? Vagus nerve;
? Kainic acid
- From:
Chinese Journal of Neurology
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the influence of vagus nerve stimulation (VNS) upon glutamate receptor (GluR) and GABA A receptor positive structures in the nucleus tract solitary (NTS) of the kainate (KA) induced epileptic rat. Methods All rats were divided into KA group, vagus nerve stimulation+KA group and the control group. The sections of the caudel medullary oblongata from each rat of every group were collected into 5 sets. The first and second sets of the sections were used to process for immunohistochemical staining by using specific rabbit and mouse antibodies against GluR5 and GABA AR and to observe the changes of GluR5 and GABA AR positive structures in the NTS. The third to fifth sets of the sections were used for control tests and Nissl′s staining. Results (1) In control group, medium density of GluR5 like immunoreactive (GluR5 LI) and GABA A receptor ? chain (GABA AR?) LI neuronal cell bodies, fibers and terminals were observed in the NTS; (2) In KA group, the density of GluR5 LI positive structures were increased, while the density of GABA AR? LI positive structures were decreased; (3) In VNS+KA group, the changes were contrary to the KA group, showing, the density of GluR5 LI positive structures were decreased, and the density of GABA AR? LI positive structures increased. Conclusion Glutamate and GABA A receptors in NTS might play important roles in KA induced epilepsy and in anti epileptic effects of VNS.
