EGFFECTS OF ATRA ON THE ACTIVITY OF LDH,ALP,? G AND SDH OF GASTRIC CANCER CELLS
- VernacularTitle:全反式视黄酸对胃癌细胞乳酸脱氢酶等活性的影响
- Author:
Yuqiang CHEN
;
Qiao WU
;
Zhengming CHEN
- Publication Type:Journal Article
- Keywords:
Retinoic acid Gastric cancer cell Engyme Differentiation Growth inhibition
- From:China Oncology
1999;0(Z1):-
- CountryChina
- Language:Chinese
-
Abstract:
PURPOSE To investigate the effects of all trans retinoic acid(ATRA) on the growth of gastric cancer cells and explore the mechanism relevant to enzyme.METHODS With MTT method the growth inhibition of gastric cancer cells was measured;the activity of lactate dehydrogenase(LDH),alkaline phosphatase(ALP) and ? glucuronidase(? G) was measured with metaplate;The activity of succinate dehydrogenase(SDH) was assayed with cytochemical method.RESULTS The growth of MGc80 3, but not MKN 45, was inhibited,which is dependent on the concentration of ATRA.The activity of LDH,ALP and ? G of Mgc80 3 was suppressed and the activity of SDH of Mgc80 3 was enhanced.But all the four enzymes′ activity of MKN 45 were not obviously changed except that introcelluar ? G activity was inhibited.CONCLUSIONS The sensitivity of gastric cancer cells to ATRA is different,which is closely related with the change of enzyme activity.
