Development of PHT PB resistant amygdala kindled rats and expression of MDR1
- VernacularTitle:大鼠耐苯妥英钠和苯巴比妥钠杏仁核点燃模型及多药耐受基因的表达
- Author:
Zheng XIAO
;
Yong YAN
;
Xuefeng WANG
- Publication Type:Journal Article
- Keywords:
Disease model, animal Antconvulsants Drug resistance, multiple
- From:
Chinese Journal of Neurology
1999;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the method of development of PHT PB resistant amygdala kindled rats. Methods By examining the effects of the antiepileptic drugs PHT and PB in a group of rapidly kindled rats,the 40 animals with different sensitivity to these drugs were selected. Then using immunohistochemistry method to determinate the expression of PGP in the brain of the rats. Results Using determination of the after discharge threshold for evaluation of PHT′s and PB′s anticonvulsant effects, 6 animals showed no increase in their ADT at repeated test trials with PHT or PB (from PHT PB resistant rats), and 6 animals exhibited reproducible increase in ADT about 100% after injection of PHT or PB (from PHT PB nonresistant rats). The immunohisto chemistry for P glycoprotein showed increased staining in capillary endothelium in the samples from PHT PB resistant rats as compared with staining in PHT PB nonresistant rats,in extensive fields of bilateral cerebral hemispheres ( P
