Establishment of detecting methods of Th1/Th2 cytokine gene
- VernacularTitle:Th1/Th2相关细胞因子基因检测方法的建立
- Author:
Jie LIU
;
Haiming WEI
;
Zhigang TIAN
- Publication Type:Journal Article
- Keywords:
Th1/Th2;
Cytokine;
Tumor cell;
RT-PCR reaction;
RNA hybridization
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: TO establish methods for sensitive, rapid, economical detection of Th1/Th2 Cytokine gene expression. Method: sixpairs of Primers specific for the IL-2, IL-4, IL-10, IL-13, IFNr and B-actin were designed. A Sensitive and rapid method for detection of Th1/Th2cytokine genes by RT-RCR was established. Meanwhile another method: the RNA hybridization with sir cDNA probes were established. Results:The RT-PCR reaction and RNA hybridization was used to test five kinds of Cytokine mRNAs expressed 20 tumor cell lines on mRNAs were expressed in eleven Cell lines;The mRNAs in eight cell lines. Conclusion:The preliminary results suggested that RT-PCR and RNA hybridizationnight be used to test Th1/Th2 cytokine gene expression.
