Expression of Proteoglycan Coreprotein Gene in Human Lumbar Discs
- VernacularTitle:腰椎间盘蛋白多糖核心蛋白的基因表达
- Author:
Shuming GU
;
Yougu HU
;
Hongjun ZHENG
- Publication Type:Journal Article
- Keywords:
Lumbar vertebrae Intervertebral disk Proteoglycans Gene expression
- From:
Chinese Journal of Orthopaedics
1996;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective\ To observe the expression of coreprotein gene in human lumbar discs in foetus and adult. Methods\ The coreprotein cDNA probe was prepared by RT-PCR. The mRNA level and distribution of coreprotein was observed in fetal and adult lumbar discs, using in situ hybridization method. Results\ 1) The highest expression lies in the lumbar nucleus pulposus of the fetal discs, less higher expression in the interior of the annulus fibrosus and the region between nucleus and annulus fibroses. 2) In adult disc tissue, few positive signals were shown. Low gene expression level of coreprotein in the subcultured disc cells was expressed. Conclusion\ The higher expression of coreprotein in fetal discs is chiefly located in the nucleus pulposus.
