THE METHODS DETECTING VASCULAR ENDOTHELIAL GROWTH FACTOR IN HUMAN BREAST CARCINOMAS
- VernacularTitle:几种检测乳腺癌组织中血管内皮生长因子表达的方法学
- Author:
Xiaoshi ZHANG
- Publication Type:Journal Article
- Keywords:
Vascular endothelial growth factor;
lmmunoblotting;
Polymerase chain reac- tion;
Immunohistochemistry
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish analysis method of vascular endothelial growth Factor(VEGF)for clinic practice.Methods: the expression of VEGF in breast carcinoma tissue is examined with immunohistochemistry, RT-PCR, Western blot and dot blot. Results: the VEGF is revealed mainly in tumor cells and extracellular matrix, molecular weight of VEGF is 46 KD, including three isoforms. In 53% of the cases the tumor tissue reveals stronger expression of VEGF than paratumor tissue. Conclusion :Dot blot may be used as a preliminary method for detecting VEGF in breast carcinoma tissue.
