THREE-DIMENSIONAL MORPHOLOGY OF HEPATOCELLULAR CARCINOMA——THE APPLICATION OF CONFOCAL LASER SCANNING MICROSCOPY IN TUMOR PATHOLOGY
- VernacularTitle:肝细胞癌癌组织三维形态学观察——共聚焦激光扫描显微镜的应用
- Author:
Wanghai ZHANG
;
Shineng ZHU
;
Shilun LU
;
Yalin HUAN
;
Peng ZHAO
- Publication Type:Journal Article
- Keywords:
HCC. 3D-reconstruction. YOYO-1, CLSM
- From:
China Oncology
1998;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
PURPOSE To investigate the application of CLSM in tumor pathology with the three dimensional reconstruction by confocal laser scanning microscopy in routine pathologic specimens of HCC, METHODS 30 ?m thick sections were cut from the paraffin-embedded tissues of HCC. hyperplasia and normal liver, stained with the DNA fluorescent probe YOYO-1 iodide and examined by CLSM to collect optical sections and 3D reconstructed images. RESULTS HCC displayed chaotic arrangement of carcinoma cell nuclei, marked pleomorphism. indented and irregular nuclear surface, and irregular and coarse chromatin texture. CONCLUSION The serial optical tomograms of CLSM can be used to create 3D reconstruction of cancer cells. Such 3D impressions might be helpful or even essential in arriving at an exact diagnosis
