THE METHYLATION OF p53 GENE IN THYROID CARCINOMA
- VernacularTitle:甲状腺癌p53基因甲基化的研究
- Author:
Gebo WEN
;
Renxian CAO
;
Qian XIANG
- Publication Type:Journal Article
- Keywords:
Genes p53 gene DNA methylation Thyroid neoplasms Mutation
- From:
China Oncology
1998;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
PURPOSE To investigate the methylation of p53 gene in human thyroid carcinoma. METHODS The DNA of 12 thyroid carcinomas and 5 adjacent tissues of thyroid carcinoma were digested by restriction endonuclease enzymes Hap I and Msp I . Polymerase chain reaction (PCR) followed by agarose gel electrophoresis was used to detect the methylation in the exon 5 of p53 gene. RESULTS 5'-CCGG-3' site was methylated at the exon 5 of p53 gene in 9 thyroid carcinomas. However hypomethylation was found to exist in 5 adjacent tissues of thyroid carcinoma. CONCLUSION Hypermethylation of p53 gene plays an important role in thyroid carcinogenesis and the mutation of p53 gene.
