EXPERIMENTAL STUDY OF PREPARATION OF AGAROSE BIOARTIFICIAL MICRO-CAPSULES
- VernacularTitle:琼脂糖微囊人工膜制备的实验研究
- Author:
Demin YU
;
Wei GAO
;
Wei YIN
- Publication Type:Journal Article
- Keywords:
Microcapsule Agarose Xenotransplantation
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The permeability of three kinds of membranes, including imported agarose (Sigma), China-made agarose and interpenetrating polymer network (IPN) made of Chinese agarose mixed with other synthetic macromolecular compound, to four substances with different molecular weights (methyl orange, Vit B12 , insulin and lysozyme) was studied. The results revealed that the solutes with molecular weights below 6 000 dalton could permeate all kinds of membranes with similar coefficient of permeability. However, the immunoisolation of IPN for such macromolecular solute, as lysozyme (14 300 dalton) was much more effective than that of other kinds, which only had limited effect of immunoisolation. These results suggest that IPN with the characteristics of efficacious immunoisolation fulfills the requirements for transplantation. 2 000-3 000 microencapsulated islets were transplanted intraperitoneally in six diabetic rats without the use of immunosuppressive drugs. On the 4th post-operative day, blood sugar dropped significantly until the 7th day, when it was in normal range. The normal level of blood sugar had remained for 30 days. The microcapsules, made of IPN, possesses a better function of immunoisolation, which may lay a foundation of treating IDDM with encapsulated porcine islet grafting.
