STUDY ON INTERFERON-? GENE-MODIFIED HUMAN TUMOR CELLS
- VernacularTitle:?-干扰素基因修饰人肿瘤细胞的研究
- Author:
Tengfei ZHANG
;
Shishu CHEN
- Publication Type:Journal Article
- Keywords:
Interferon-? gene Expression of HLA- Ⅰ,Ⅱ Tumorigenecity
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The whole length cDNA of human interferon-?(IFN-?) gene including signal peptide was constructed to retroviral vector pLXSN and then packaged with PA317. A Human Hepetoma Cell Line(HHCL) and a human gastric cancer cell line (MKN-45) were infected by using supernatant containing retroviral vector with IFN-?. the gene-modified tumor cell. were isolated by G418 selection. Some changes were found in cell cycle, expression of HLA class Ⅰ and classⅡ, and tumorigenecity. Elevated immunogenecity and abrogated rumerigenecity suggest a means for generating gene-modified tumor cell vaccine. for the treatment of cancer.
