DETECTION OF IDDM SUSCEPTIBILITY IN SHANGHAI HANS WITH HLA-DQA, OLIGONUCLEOTIDE PROBES
- VernacularTitle:HLA-DQA_1位点寡核苷酸探针检测上海地区汉族IDDM易感性
- Author:
Rong SUN
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction Dot-blot hybridization Allele IDDM HLA-DQA1 Polymorphism
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
With polymerase chain reaction and dot-blot hybridization with allele-specific oligonucleoade probes,we analyse DQA1 gene of 42 insulin-dependent diabetes mellitus (IDDM) and 40 normal controls (the Han nationality in Shanghai). We found that the frequency of DQA1 * 0301 and DQA1 * 0501 allele in IDDM patients was greatly increased, while the frequency of DQA1 * 0103 and DQA1 * 0201 allele was significantly decreased as compared with the controls. The result indicates the contribution of the DQA1 * 0301 and DQA1 * 0501 alleles to IDDM susceptibility in Han population in Shanghai. Our results also provide confirmation of the role of DQA1 * 0103 and DQA1 * 0201 to IDDM resistance in the same population.
