Experimental model of human interleukin-6 gene therapy and its in vivo interleukin-6 level
- VernacularTitle:成纤维细胞介导的人白细胞介素6基因疗法的建立及其体内白细胞介素6分泌水平的实验研究
- Author:
Xuetao CAO
- Publication Type:Journal Article
- Keywords:
Interleukin-6;
Gene therapy;
Fibroblast cells;
Implantation;
Gene transfer
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
In the present study,we have established an experimental model of human IL-6 gene thera-py with fibroblasts as cellular delivery and then observed its in vivo IL-6 level at different time.650-bp-long hIL-6 eDNA was inserted into Xho Ⅰ site of expression vector BCMGNeo carryingNeo~R gene and the resulting recombinant expression vector BCMGNeo-IL-6 was identified by re-striction enzyme digestion.BCMGNeo-IL-6 was transfected into NIH3T3 fibroblast cells by cal-cium phosphate coprecipitation method.A fibroblast cell clone secreting IL-6 activity in the cul-ture supernatants,and then analysed by Southern blot.The cell clone was expanded in vitro,en-capsulated into collagen and implanted i.p.into mice.Serum IL-6 could be detected even after15 days implantation.These results demonstrated that fibroblast cells could mediate in vivotransfer and efficient expression of hIL-6 gene,suggesting that this kind of gene therapy is reli-able.
