Detection of anti-RNA antibody by dot-immunogold silver staining and its clinical significance
- VernacularTitle:斑点免疫金银染色检测RNA抗体及其临床意义
- Author:
Xiaohui JI
- Publication Type:Journal Article
- Keywords:
Dot immunogold-silver staining(IGSS);
Ribonucleic acid(RNA);
Antibody;
Autoimmunodiseaese(AID)
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Anti-RNA antibodies(ARNA)were determined by dot-immunogold silver staining(Dot-IGSS)in 280 clinical serum samples.144 of these sera were tested simultaneously by ELISA ascontrast.The coincident rate of the detection results obtained from Dot-IGSS and from ELISAwas 91% and the difference between these results was insignificant in statistics.Moreover,therewas a positive linear correlation between the results Of quantitative analysis by the two methods.The positive rate of ARNA was 46.6% in group of autoimmunodiseases(AID),while 5.1% inthe group of non-AID and normal persons and ARNA could be regarded as another lead forscreening AID,especially for ANA-negative patients.
