Study on the Isolation and Culture of Vital CEC from Peripheral Blood of Traumatic Patients
- VernacularTitle:创伤病人循环内皮细胞体外培养的研究
- Author:
Yingbo YANG
- Publication Type:Journal Article
- Keywords:
Traumatic patients Cireulating endothelial cells Culture
- From:
Chinese Journal of Trauma
1993;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Vital circulating endothelial cells (CEC) from peripheral blood of traumatic patients were successfuly isolated and cultured in vitro with a centrifugation method on density gradient of percoll. After culturing for 72 hr in vitro, the cells formed several dense groups. The technique of alkaline phosphatase monoclonal antioalkaline phosphatase (APAAP) found that the cells originated from vaslular endothelial cells (VEC). The results indicated that trauma could cause the detachment of VEC, and the culture of vital CEC from peripheral blood of traumatic patients may enlighten an attracting perspective.
