An Experimental Study on Injury and Recovery of Rabbit Corneal Endothelial Cells after Blast
- VernacularTitle:冲击波对兔角膜内皮细胞损伤及其修复的实验研究
- Author:
Yingxue ZHANG
- Publication Type:Journal Article
- Keywords:
Corneal endothelium Tissue culture Na/K ATPase Morphology Blast injury Recovery
- From:
Chinese Journal of Trauma
1990;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
After blast injury, the morphologie and ultrastructural differentiation of the rabbit corneal endothelium in primary culture was evaluated and compared with the Na/K ATPase activity that occoured during wounding and healing. Morphologie and ultrastructural changes were investigated by light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The endothelial injury and recovery after blast can be described as a three—stage process. Stage one was characterized by a desquamation, degeneration and necrosis of endothelial cells and a low activity of Na/K ATPase; In stage two, some surviving endothelial cells appeared to migrate and divide and the activity of Na/K ATPase began to increase. In stage three, surviving endothelial cells displayed a flattened configuration consisting of polygons and a high ATPase activity.
