ANALYSIS OF ?-TOCOPHEROL CONTENTS IN SEA-BUCKTHORN OIL BY REVERSED PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
- VernacularTitle:沙棘油中?-生育酚含量的反相高效液相层析分析法
- Author:
Qiang FU
;
Qunyin YANG
;
Guangde YANG
- Publication Type:Journal Article
- Keywords:
sea buckthorn oil;
?-Tocopherol;
reversed phase-high performance liquid chromatography
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
For controlling the quality of sea buckthornoil, a reversed phase-high performance liquid chro-matography (RP-HPLC) method was establishedto determine the a-Tocopherol contents. Aftersaponated, the sea buckthorn oil samples were ex-tracted by ethyl ether. The extraction liquid wascondensed and tben dissolved in anhydreousethanol for liquid chromatographic analysis. Chro-matographic conditions were as follows: ZORBAXODS column (4. 6 ? 150 mm); methanol as mobilephase (1. 0 ml/min); the wavelength of UV detec-tor: 280 nm. Three branches of sea buckthornseed oil and three branches of fruit oil which wereproduced by Yongshou County, Shaanxi Provincewere determined. The average contents of a-Toco-pherol were 989 mg/kg, and 1745 mg/kg, respec-tively. The recoveries of this method were 98. 6%(for seed oil), 102. 2% (for fruit oil) with CV lessthan 5. 44%.
