Characteristics of Natural transformation of C_3 components and regulation of rIFN-? and Sizofiran on pro-C_3
- VernacularTitle:补体C_3的自然转换特征及rIFN-?和SPG对C_3的调节
- Author:
Huimin REN
- Publication Type:Journal Article
- Keywords:
C_3 degradation and regulation;
pro-C_3;
rIFN-?;
SPG
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
1.Shanghai Institute of Physiology,Chinese Academy of Sciences.Shanghai 200031.2.Central Research Laboratories,Kaken pharmaceutical Co.,Ltd.Tokyo 107.3.Department of Gynecology,Cancer Institute Hospital.Tokyo 117.The natural transformation of C_3 components and regulation of rIFN-? and/or SPG on pro-C_3 were observed by immuno-blotting analysis.Under the condition of sera stored at 4℃,the pe-riod of transformation from pro-C_3 to C_(3(?)) was about 18 days.The highest level of fragment C_3and C_(3b) appeared on day 8 during the degradation of pro-C_3.Level of pro-C_3 could be enhancedby administration of rIFN-? and/or SPG,and the most potent means was to take the method ofrIFN-? and SPG in combination.These results were suggested that so called the third compo-nents of complement in vivo was in pro-C_3 form in reality,and most reagent was to affect on thesynthesis of pro-C_3 instead of other C_3 components.In addition,all obtained results about the lev-els of C_3 components should be considered as an“instantaneous result”due to the C_3 componentsvaried with the store time-course of sera.
