The relationship between the neuroednocine polypeptide 7B_2 and T lymphocytoe
- VernacularTitle:神经内分泌多肽7B2与T淋巴细胞间的关系
- Author:
Yiping XU
- Publication Type:Journal Article
- Keywords:
Neuroendcrine polypeptide 7B_2;
Active Trosettes;
FACS
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The data presented in this work demonstrated that the neuroendocrine polypeptide 7B2(residues 1-150) can decrease active T rosettes formation,at the concentration of 300-500 pg/ml,using 12 different porcine thymocyte preparations,or 10 different human T lymphocytepreparations.Synthetic 7B2 (23-39) showed a similar inhibitory effect,but not with 7B2 (117-128) or 7B2 (141-150).50% of human and porcine thymocytes showed bright fluorescent spotson cell surface by immunofluorescent microscope and FACS analysis after incubation of the thy-mocytes with rabbit anti-TB2 (23-39) antibody,then fluoresceincoupled goat anti-rabbit IgG(second antibody).Furthermore,cell surface fluorescent staining was abolished by preincubationof thymocytes with 0.5% trypsin,but was only slightly reduced by heat pretreatment,suggest-ing that the observed binding was due to 7B2 interaction with a thymocyte surface protein.
