Analysis of the Target Antigen Recognized By The Monoclonal Antibody (2H3)
- VernacularTitle:抗激活小鼠T细胞抗原单克隆抗体(2H3)识别的靶抗原分析
- Author:
Linlin ZHAO
;
Qiuzao HE
- Publication Type:Journal Article
- Keywords:
Acfivated mouse T cell surface anfigen;
IL-2 recepfor;
anti-IL-2 receptor monoclonal antibody
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The McAb 2H3 to different target cells was investigated by using an indirect ELISA meth-ods. The result showed that the 2H3 could bind to the ConA actiuated mouse T lymphoblastsand CTLL-2 cells (an IL-2 dependent T cell line)but not to the resting mouse splenocytes,lymph node cells and thymocytes. The binding of FITC-anti-IL-2R to the activated mouse spleencells and CTLL-2 cells was seen as tested by immunofluorescence analysis. This binding reactionwas blocked when the target cells were previously treated with 2H3, but the binding reaction ofFITC-anti-Ia McAb to these target cells couldn't be blocded by 2H3. Further analysis of the tar-get antigen by using Western-Blotting technigue showed the molecular weight of this antihenrecognized by 2H3 was of 50～60KD in reducing condition. According to these data, target anti-gen recognized by the McAb 2H3 may be the IL-2R molecule(P_(55) mole cule)on the cell surface.
