DNA-fluorometric assay for cytotoxicity and NK cytotoxic activety of peripheral blood lymp?o cytes from cancer patients
- VernacularTitle:细胞毒效应的DNA荧光测定法测定恶性肿瘤患者外周血NK细胞活性
- Author:
Jianming HUANG
- Publication Type:Journal Article
- Keywords:
NK cytotoxic activity;
Cytotoxicity;
Malignant neoplasm;
DNA-fluorometric assay
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
On the fluorescent reaction of ethidium bromide binding double-stranded DNA after cell ly-sis has been used to develop a rapid fluorometric assay for cytotoxicity. By using this technique,the cytotoxic activity of effector cells against target cells can be assayed by measuring the fluo-rescent intensities of the residual target cell DNA and the control target cell DNA. Our experi-mental results show that the fluorescent intensity of the uninjured, residual K562 cell DNA is ininverse relation to the cytotoxic activity of NK cells after incubation of peripheral bloed lympho-cytes with K562 cells. for 20 hr, at a different ratio of effector cell to target cell. We detected thecytotoxic activities of NK cells of perpheral blood lymphocytes from 34 normal individuals and 30cancer patients, and their mean percentages of NK cytotoxicity were 51.96?6.62% and 25.02?10.83% respectively, and had much significant deviation statistically(P
