~(32)P-POSTLABELING TEST OF DNA ADDUCTS FORMED IN THE LIVERS OF RATS TREATED WITH CARCINOGEN
- VernacularTitle:致癌物染毒大鼠体内DNA加合物的检测
- Author:
Jianhua YI
;
Yuhua CHEN
;
Li WANG
- Publication Type:Journal Article
- Keywords:
carcinogenicity;
benzidine;
DNA adducts;
~(32)P-postlabeling assay
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
DNA in the livers of rats was isolated 24 h af-ter oral administration of benzidine, Congo red andEvan's blue, DNA adducts in the livers of ratswere investigated by a ~(32)P-postlabeling assay. Thelevel of DNA adducts in the livers of rats after oraladministration of benzidine, congo red andEvan'blue were 18. 17, 1. 03 and 1. 74?mol/kgDNA respectively. The results showed that themetabolites of the benzidine derivative azo dyes canform adducts with DNA in the livers of rats.These DNA adducts probably are one of the rea-sons that the incidence of the bladder cancer rosein the people group who are only exposed to benzi-dine derivative azo dyes.
