Preparation and primary application of human c—fos antisense RNA
- VernacularTitle:人原癌基因c—fos反义RNA制备及初步应用
- Author:
Baoxue YANG
;
Guizhen YANG
- Publication Type:Journal Article
- Keywords:
Antisense RNA;
Oncogene
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The cDNA fragment of human c—fos oncogene has been obtained by a reverse transcription—DNA poly-merase chain reaction.Then the cDNA was ligated into pGEM3Zf(+)plasmid cut with Sma Ⅰ.with the re-combined plasmid,c—fos antisense RNA was synthesized in T7 RNA polymerase system.The primary appli-cation of anti-sense RNA is also studied in this article.
