A bioassay method for human Granulocyte—Macrophage Colony stimulating factor with DMSO—induced HL60 cell line
- VernacularTitle:应用DMSO诱导的HL60细胞检测HGM—CSF生物学活性
- Author:
Chuanshu HUANG
;
Boquan JIN
- Publication Type:Journal Article
- Keywords:
GM—CSF;
bioassay;
GM—CSF;
dependent cell line;
leukemia cell lne
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Based on the high level of human granulocyte—macrophage colony—stimulating factor(HGM—CSF)re-ceptor of HL60 cell line upon induction by DMSO,we developed a hioassay method for HGM—CSF with ahigher specificity and sensitivity.The sensitivity of this assay reached to 0.3ng/ml by selecting the optimalHL60 cell—induced time,cell density and GM—CSF—stimulated time.With this assay,we are able to quantifythe level of rHuGM—CSF as well as conditioned media from human bladder carcinoma cell line U5637 screatinga high level of GM—CSF.
