A Novel Solid—phase Competitive TRFIA for Serum Progesterone
- VernacularTitle:新型固相竞争性时间分辨荧光免疫检测血清孕酮方法的建立
- Author:
Ping GAO
- Publication Type:Journal Article
- Keywords:
Progesterone TRFIA Biotin—Streptavidin
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
The acticle describes a time—resolved fluoroimmunoassay for serum progesterone,based ona competitive solid—phase antigen technique。Progesterone—BSA conjugates are coated onto thewhite opaque microtitration wells。Anti—progesterone antibody is biotinylated.Eu~(3+) are labelledto streptavidin as the tracer.The assay standard curve covers a range of 0.1—100ng/ml.Thelowest detecting limit is 0.05ng/ml。The intra and inter C?V s are 6.45% and 8.93% respec-tively.The average recovery is 89.5%.40woman samples are measured with RIA and presentTRFIA and the results are satisfactory,giving a correlation coefficient of 0.9619.The perfor-mance charicter—istics of the assay are discussed and compared with other known immunoas-says.
