Studies on the expression of human interleukin 2gene in yeast and its fermentation
- VernacularTitle:人白细胞介素2基因在酵母菌中的表达及发酵研究
- Author:
Jiang CAO
- Publication Type:Journal Article
- Keywords:
Human interleukin 2;
Yeast;
Recombinant;
Transformantion;
Hybridization;
Expression;
Fermentation
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
We transformed a E.coli—yeast shuttle plasmie containing human interleukin 2 gene into a yeast strain Saccharomyces cerevisiae 8534—8c,then hybredized the transformants with another yeast strain Saccharomyces cerevisiae CSH 83—L to get diploid hy brids The hap Joid transfor-mants and diploid gybreds were compared for their expression level of recombinant interleukin 2.We also studied the gybrids'rIL—2 expression under various conditions and its fermentation.
