Covalent attachment of immunogl oblins to.liposomes via gangliosides
- VernacularTitle:脂质体与抗体共价偶联的过碘酸钠法
- Author:
Bing WANG
- Publication Type:Journal Article
- Keywords:
Liposome;
Periodate method
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
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Abstract:
A efficient and convenient method for covalent binding of antibodies to liposomes was de-scribed.Antibodies were attached to the aldehye groups on the gangliosides by the periodate ox-idation to form schiff—base and the complex was stabilized by reduction.Separate the liposome —IgG complex from unbound IgG using discontinous dextran T40 gradient centrifugation.AKP and HRP were coupled to liposomes,and both enzymatic activity was remained more than 80%.and compared this with glutaraledhyed method.
