AMPLIFYING cDNA OF GLUTATHIONE PEROXIDASE BY RT-RCR
- VernacularTitle:用RT—PCR扩增人谷胱甘肽过氧化物酶的cDNA
- Author:
Guangyuan LI
;
Xiaojun YAN
;
Yingyun REN
- Publication Type:Journal Article
- Keywords:
glutathione peroxidase;
gene;
cDNA;
RT—PCR
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The glutathione peroxidase cDNA has been acquired by reverse transcriplate-polymerase chain reaction (RT-PCR). mRNA was isolated from the culture liver cancer cells firstly, then trancriplate if into cDNA with AMV reverse transcriptase. By using this cDNA as PCR template and two synthetic special primers, we get about 600 bp DNA fragment after 35 circles. The result is identical with our expectation.
