THE COMPARISION OF IN SITU HYBRIDIZATION BY USING ~3H WITH DIGOXIGENIN LABELED TNF PROBE
- VernacularTitle:用~3H和地高辛配基标记TNF—? cDNA探针进行原位杂交的比较
- Author:
Tianju LIU
;
Lusheng SI
;
Juxiang YANG
- Publication Type:Journal Article
- Keywords:
~3H labeled TNF probe;
Digoxigenin labeled TNF probe;
in situ hybrdization
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The TNA mRNA expression in 33 samples of cervical carcinoma and 28 samples of condyloma have been observed by using ~3H and Digoxigenin labeled TNF—? cDNA probes and in situ hybridization technique, we found that the results of in situ hybrdfzation with these two kinds of labeled probes were alike, the sensitivity with ~3H labeled probe was slightly higher than that with Digoxigenin labeled probe. Nevertheless, the nonisotope probe would be used more and more in future because of its safety, rapidity and convenience in work.
