cDNA Library screening by a DNA polymerase chain reaction technique and the isolation of encoding region cDNA of human interleukin 8
- VernacularTitle:以DNA聚合酶链反应筛选cDNA文库及人白细胞介素-8全编码区cDNA的钓出
- Author:
Yongli YU
;
Guizhen YANG
;
Yin WU
- Publication Type:Journal Article
- Keywords:
DNA polymerase chain reaction;
cDNA library;
Interlieukin 8;
Fibroblast
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Using human acidic fibroblast growth factor Specific primers we tested the technique of screening cDNA library by a DNA polymerase chain reaction.With the method,we have suc-cessfully isolated encoding region cDNA of human interleukin 8 from both human dermal fibrob-last and PMA stimulated U937 cell cDNA libraries.
