Preparation,Characterization and application of monoclonal antibodies to recombinant human interferon—?2a
- VernacularTitle:重组人?2a型干扰素单克隆抗体的研制、特性鉴定及初步应用
- Author:
Yong ZHU
- Publication Type:Journal Article
- Keywords:
Recombinant human interferon—?2a;
Monoclonal antibody;
Epitope specificity;
Neutralization;
Affinity purification;
Sandwich ELISA
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In this report,five mouse hybridoma cell lines secreting monoclonal antibodies to recombi-nant human interferon—?2a(rHu IFN—?2a)were raised.All the monoclonal antibodies with IgGl subclass react with rHu IFN—?2a detected by indirect ELISA with the titers ranging from 1:320,000 to 1:10,240,000 but have no cross reactivity with rHu IFN?l,? and proteins from recombinant E.coli.Two of monoclonal antibodies,designated 3F1 and 5G10,have the ability to neutralize antiviral activity of rHu IFN—?2a.By a competitive ELISA the five monoclonal anti-bodies can be divided into three categories based on their epitope specificities.A sandwich ELISA established by employing 3F1 and 5G10 monoclonal antibodies could detect rHuIFN-?2a or rHuIFN-?2b as few as 60pg/ml?Moreover,3F1 monoclonal antibody displayed a satisfactoryproperty in affinity chromatography for rHuIFN?2a,which is able to match a monoclonal anti-body NK2 from CellTech Corporation.
