Studies on the requirements for growth of Epstein-Barr Virus thransformed human lymphoblastoid B cells at low cell density
- VernacularTitle:EB病毒转化的人B淋巴母细胞低密度培养条件的研究
- Author:
Chen SUN
- Publication Type:Journal Article
- Keywords:
Epstein-Barr virus;
Human B lymphoblastoid cell;
low density culture;
Haemorrhagic fever with renal syndrome virus;
Cloning
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
We obtained human lymphoblastoid B cells secreting human antibodies IgM and IgG to HFRS virus by Epstein-Barr virus(EBV)transfomation of PBMC.Conditioned media from the supernatant of a human fibroblast cell CRL1506 culture and irradiated CRL1506 cells as feeder cells could markedly promote the growth and antibody produotion of the human B lymphoblastoid cells at low cell density.It was shown that conditioned media contained a high level of interleukin 6(IL-6) .These results would be very useful for human monoclonal antibody technique including improving human B lymphoblastoid cell growth at low cell density and enhancing cloning efficiency andantibody production.
