FUSION OF HEPATOMA CELL
- VernacularTitle:肝癌细胞的融合方法探讨
- Author:
Xiaoming OU
;
Junming WANG
;
Chen HUANG
- Publication Type:Journal Article
- Keywords:
hepatoma cell;
cell fusion;
polyethylene glycol-induetxt;
centrifugation
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Both molecular weight 6000 and 1000 polyethylene glycol (PEG) have been used to induce thc fusion of hepatoma cell by two methods of centrifugation and stillstand. The results showed that 50% PEG of molecular weight 1000 and centrifugation increase the percentage of fused cells. The procedure of cell fusion has been examined. The cytoplasm has been fused when the cells start fusing in one hour and a half, and the neucleus start fusing in two horus and a half.