MEASUREMENT OF HUMAN GROWTH HORMONE: DEVELOPMENT AND VALIDATION OF A SENSITIVE AND SPECIFIC ASSAY
- VernacularTitle:人生长激素LAB-ELISA测定方法
- Author:
Weiqun HUANG
- Publication Type:Journal Article
- Keywords:
Biotin/Avidin Growth hormone Dwarf ELISA
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
The determination of human growth hormor.e (hGH) in urine by means of LAB-ELISA technique was described. The major factors affecting the Sensitivity of LAB-ELISA were studied systematically. The optimized conditions have been established. The sensitivity of LAB-ELISA is at the level of pg/ml. There were little cross binding with human prolactin, thyroid-stimulating hormone, follicular-stimulating hormone, and luteinizing hormone. Human growth hormone levels in overnight urine in 50 normal children and 11 cases of GH-deficiency (GHD) were measurejl by the LAB-ELISA technique. Results of recovery test (recovery rate 100 ?1.4%) and precision test (intra-assay CV 4.72-12.00%, inter-assay CV 4.89-11.17%, and bias 0.20-12.00%)indicate that the method is highly specific,precise and stable.
