Study on proliferation of HPBL and expression of CD2 mRNA
- VernacularTitle:人外周血淋巴细胞激活与CD2 mRNA 表达
- Author:
Bo GUO
- Publication Type:Journal Article
- Keywords:
CD2 mRNA;
T cell;
PHA;
CD2;
CD3 McAbs
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The effects of various stimuli on activation and proliferation of human peripheral blood lymphocytesHPBL)nd expression of CD2 mRNA were studied.The total RNA was extractedfrom the activatedHPBL and Northern blot ssay of these RNA samples were carried out with CD2 antisense RNAprobe prepared.It was shown that anti—CD3 Abs RW2-4B6 and RW2-8c8,PHA,and a calfthymic peptide preparation could all induce the activation and roliferation f HPBL and high levelexpression of CD2 mRNA.It was interresting to note that anti-CD3 McAb RW2-8c8 when usedtogether with antiT11_2 and anti-T11_3,inhibited the activation of HPBL and resulted in very low evelexpression of CD2 mRNA.The possible explaination of the phenomenon was discussed.
