The influence of Sodium Butyrate on hybridoma proliferation and antibody production
- VernacularTitle:丁酸钠对杂交瘤细胞增殖及抗体分泌的影响
- Author:
Hao DING
- Publication Type:Journal Article
- Keywords:
Hybridoma culture;
Sodium Butyrate;
McAbs production
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Using different concentration of sodium butyrate in RPMI 1640-20 or serum-free medium to culture hybridoma cells which secreted monoclonal antibodies (McAbs) against tissue-type plasminogen activator (t-PA). The results indicate that 0.25mmol/L-0. 5mmol/L of sodium butyrate could incite hybridoma cells to produce McAbs and had no inhibition on hybridoma cell growth. The maximum titer of McAb was 1:2500, 2-4 fold more than control. Sodium butyrate may be as an additive to enhance the ability of hybridoma secreting McAbs.
