Establishment of a sandwich ELISA method for measuring soluble IL-2 receptor
- VernacularTitle:一种检测可溶性白细胞介素2受体夹心法ELISA的建立
- Author:
Chen SUN
- Publication Type:Journal Article
- Keywords:
Soluble IL- 2 receptor (sIL- 2R);
Sandwich ELISA;
Monoclonal antibody (McAb)
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Two kinds of mouse monoclonal antibodies which recognize distinct epitopes of the IL-2 receptor (a chain) were used to establish a sandwich ELISA for measuring soluble IL-2 receptors. The results indicated that this method is sensitive and specific for detecting soluble IL-2 receptors of cell culture supernatant and serum from both healthy donor and patients with leukemia. Therefore this sandwich ELISA method is useful for basic and clinic immunology research.
