Studies on different conditions for denaturing DNA and RNA
- VernacularTitle:核酸变性条件的探讨
- Author:
Zhongbin LIU
;
Guizhen YANG
- Publication Type:Journal Article
- Keywords:
DNA;
RNA;
Denaturing
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The conditions for denaturation of DNA and RNA were invertigated. The results show that one minute to heat DNA at 100鈩?is sufficient for denaturation of DNA (several hundreds bp to 23kb). DNA degraded more obviously with longer neating time and the degradation decreased when DNA was dissdved in TE buffer. But no difference of degree of RNA degradation was found in there three conditions for RNA denaturation.
