Establishment of hybridoma cell lines secreting monoclonal antibodies against alkaline phosphatase and their applications on immunohistochemistry research
- VernacularTitle:分泌高效价抗碱性磷酸酶McAb杂交瘤系的建立及其在APAAP免疫组化研究中的应用
- Author:
Ning ZHAO
- Publication Type:Journal Article
- Keywords:
Monoclonal antibody;
Hybridoma;
APAAP;
Immunohistochemistry technique
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
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Abstract:
BALB/c mice were immunized by multi-route injections of commencial alkaline phosphatase over a perioh of three months. After 3 days of the last intravenous boost spleen cells were fused with sp2/0 cells by using PEG and hybridoma supernatants were screened by reverse ELISA. Two hybridoma cell lines secreting high titer monoclonal antibodies to alkaline phosphatase were established. Satisfactory results were obtained by alkaline phosphatase antialkaline phosphatase (APAAP) immunohistochemistry technique in detecting CD antigens, viral antigens, Tumor antigens and bacterium fimbriae antigens.
