Measurement of soluble IL-2 receptor by sandwich ELISA with monoclonal and polyclonal antibodies
- VernacularTitle:单克隆与多克隆双抗体夹心法测定血清可溶性IL-2受体
- Author:
Ning FU
- Publication Type:Journal Article
- Keywords:
soluble IL-2 receptor;
sandwich ELISA
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
In this work, a simple and sensitive assay for soluble IL-2 receptor (sIL-2R) was established. The main procedures are as follows: the microplate was coated with anti-Tac monoclonal antibody (McAb); after sIL-2R in samples bound with the McAb, the rabbit anti-IL-2R serum was added;and HRP-goat anti-rabbit IgG was used to display and amplify this binding. The sensitivity of this method is about 100u/ml, which is near that in "Cellfree IL-2 Receptor Test Kit".
