Semi-quantitative determination of HLA-DR antigen expression on cell surfaces with dot-ELISA
- VernacularTitle:单克隆抗体Dot-ELISA半定量检测细胞表面HLA-DR抗原
- Author:
Xiaojuan ZHAO
;
Jiying ZHANG
- Publication Type:Journal Article
- Keywords:
Dot-ELISA;
HLA-DR antigen;
Flying-spot scanning
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Using HB55 McAb, we detected HLA-DR antigens on Daudi, H18, PBL and K562 cells by dot-ELISA. Results corresponding to situation of the antigen expressions on various cell lines were obtained: colour reaction in Daudi, H18 cells and PBL was positive in different degree and K562 sample presented negative reaction. Chromatogram peaks were printed by Dual-Wavelength Flying-Spot scanner CS-9000 (SHIMADZU). Areas and high values of peaks, meaning size and colour of spot respectively, were increased with cell concentration. Both cell concentration and volume of cell suspension did not influence the diffusion of sample. An analysis for the antigen expression on cell surface and its dynamic change can be supplied by the technique.The study on quantitative assay of MHC class Ⅱ antigens on cell surface are still going on.
