Establishment of radioimmunoassay for detecting Neuropeptide Y
- VernacularTitle:神经肽Y放免分析法的建立
- Author:
Jingping YANG
;
Guizhen YANG
- Publication Type:Journal Article
- Keywords:
Neuropeptide Y;
Radioimmunoassay
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
A simple specific and sensitive radioimmunoassay for ne(?)ropeptide (NPY) in plasma and tissue extracts was developed. There was no appreciable cross-reactivity with related neuropeptides. The minimum detectable NPY in plasma and tissue extracts was 150-200pg/ml and average recovery was 85-90%. Reverse-phase HPLC reveals that chromatographic charecterization of NPY-Li in plasma and tissue extracts was very similar to that of standard NPY.