Establishment of radioimmunoassay system for detecting corticotropinreleasing
- VernacularTitle:促肾上腺皮质激素释放因子放射免疫测定法的建立
- Author:
Zhonghui LIU
;
Guizhen YANG
- Publication Type:Journal Article
- Keywords:
Corticotropin-Releasing Factor (CRF);
Radioimmunoassay (RIA);
Hypothalamus
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The porcine CRF partly purified according to Vale's method used an immunogen to prepare the rabbit anti-CRF serum. The specific CRF radioimmunoassay was developed with the antiserum and ~(125)I-h-CRF. The antiserum revealed 20% tracer binding at 1: 1600 working dilution and was no cross-reactivity with relative peptides in RIA. The lower limit of detection of standard CRF was 25-50pg/ml in RIA. The concentration of CRF in plasma and brain tissue was measured by this method. The results are consistent with that of Sapolsky et al. Therefore the CRF RIA can be used for CRF detection in plasma and tissue extracts.
