Electroporation transfer of human leukocyte differentiation antigenes CD_4, CD_(19) genes into mammalian cells
- VernacularTitle:电脉冲介导基因转移法在真核细胞表达CD_4和CD_(19)抗原
- Author:
Jianan WANG
- Publication Type:Journal Article
- Keywords:
Electroporation;
Human leukocyte differentiation antigen;
Cos cell;
Monoclonal antibody
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Human leukocyte differentiation antigens CD4 and CD19 gene were transfected into Cos cell line by electroporation method. Indirect immunoflucence and APAAP immunocytochemical staining shows that transient expression and highefficiency transformation of CD4, CD19 genes was obtained. It can provide a new way for reserch on molecular strcture and function of IL-2 receptor gene, identification and screen of Anti-IL-2 receptor McABs.
