Expression of recombinant human interleukin 8 in E.coll and its functional identification
- VernacularTitle:人重组IL-8在大肠杆菌中表达及其活性检测
- Author:
Baohong ZHOU
- Publication Type:Journal Article
- Keywords:
Recombinant human interleukin 8;
Genetic engineering;
Chemotactic activity
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
By using genetic engineering techniques, we had successed in the expression of recombinant human interleukin 8 (rhIL-8)in E. col(?) IL-8 cDNA insert into the plasmid pGEM-IL-8 was subcloned into expression vector pMS316, and IL-8 fusion protein was produced in E.coli with high efficiency, The purified recombinant protein exhibited chemotactic activity for mouse neutrophils both in vive and in vitro. It showed that a fragment of the human IL-8 had no influence on the chemotactic activity of IL-8.
