pL Promoter controlled expression of human interleukin-4
- VernacularTitle:pL启动子控制的人白细胞介素4表达
- Author:
Jide TIAN
- Publication Type:Journal Article
- Keywords:
Human rIL-4;
Gene expression;
pL Promoter
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Utilizing higher expressing vector and a synthetic oligonucleotide linker,a new expressing clone, pBMhIL 4, has been constructed in our laboratory. The intact human rIL-4 protein molecule was expressed under the control of pL promoter in E. coli. The gene encoding protein was expressed in the plasma of E. coli in the form of inclusion bodies, the expressed IL-4 constituting about 5-10% of the total cellular proteins by SDS-PAGE analysis. The molecular weight of human rIL-4 was about 15KD. After extraction and renaturation, the yieldedsoluble human rIL-4 exhibited biological activity. 1?10~6 units of human IL-4 were produced from 1 liter of bacteria extracts assassed by the assay of its TCGF activity. Using 3.5M Guanidine hydrochloride and 0.25% Triton X100 to lyze inclusion bodies resulted in higher yield and higher biological activity.
