Study of JCBI spontaneously producing a suppressor factor
- VernacularTitle:胶质瘤细胞自发分泌免疫抑制因子的研究
- Author:
Zhiqing HU
;
Guizhen YANG
- Publication Type:Journal Article
- Keywords:
Human glioma;
Inhibiting factor;
Cell line
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
JCBI is a glioma eell line established in our department. JCBI cells spontaneously produce a factor inhibiting blastogenic responses of mouse thymocytes and the IL-2 dependent T cell growth of mouse spleen lymphoblast, without inhibiting spontaneous proliferation of mouse thymocytes. The inhibiting activity is high after 72 hours of passage. Physicochemical characterization shows that the factor is nondialyzable. partially inactivated at 37℃ 48 hours and almost inactivated at 56℃ 30 min. But the factor is stable at 4℃ for 2 weeks and in freeze-thawing. The molecular weight of the factor is approximately 10 kd as estimated by gel filtration. The factor may contribute to the impaired immunosurveillance and to the cellular immunodeficiency state in the patients.
