The establishment of 3 cell strains secreting anti-acute non-Iymphocytic Ieukemia M_2-type McAb and their characterization
- VernacularTitle:分泌抗急性非淋巴细胞白血病M_2型单克隆抗体细胞株的建立及其特异性
- Author:
Quanyi ZHANG
- Publication Type:Journal Article
- Keywords:
Acute non-lymphocytic leukemia Mi-type;
Monoclonal antibody hybridoma
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
By the application of hybridoma technique we have established 3 cell strains designated as 1 D 1, 1 C 2 and 1 D6, which all the strains secrete anti-acute nonlymphocytic leukemia M2-type monoclonal antibodies individually. These strains secrete the McAb steadily after passed on propagation over 11 months. The McAb secreted by these cell strains all give positive reactions with the leukemia cells from actue non-lymphocytic leukemia M2-type pationt, but not with cells from M1, M3, M4, and M5 type patients. These McAb also can not react with cells from chronic myelocytic leukemia and its acute transformation phase and acute lymphocytic leukemia patient. The McAb can not react with white cells from normal adults too. From the above metioned we may conclude that the McAb secreted by these cells possess a remarkable specificity. Therefore it is applicable for the immuno-typing diagnosis of acute non-lymphoc yt ic leukemia and researches on target directed therapeutics.
