The preparation of human IL-2R cDNA probe and its application in the detection of mRNA levels
- VernacularTitle:人IL-2R?cDNA探针的制备及其在mRNA检测上的应用
- Author:
Enzi JIANG
;
Guizhen YANG
- Publication Type:Journal Article
- Keywords:
IL- 2 R ? cDNA probe;
Dot hobridization
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
We obtained human IL-2R cDNA 673 bp fragments from plasmid pJSB15 and prepared ?-32 p-IL-2 R cDNA probe. Dot hybridizations of RNA extracted from human lymphocytes were done by using the probe. The results showed that PHA or TPA could induce IL-2 R mRNA, respectively; and the synergistic effects were seen when used them together. IL-2R mRNA could be expressed spontaneously in many kinds of leukemic lymphocytes isolated freshly from PBL of leukemic patients.
