Utllizition of ELISA in determination of monoclonal antibody affinity constant in ascites
- VernacularTitle:改良ELISA测定腹水中单克隆抗体的亲和常数
- Author:
Xi WANG
- Publication Type:Journal Article
- Keywords:
Monoclonal antibody;
ELISA;
Antibody affinity
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Antibody affinity influences the ant igen-antibody reaction in vivo and in vitro. It is important to routinely determine the antibody affinity in order to elucidate the impacts of this antibody characteristics on the immunoassay and the pathogenetic mechanisms of human diseases. In this paper, a simple method (modified Friguet' s) was reported to directly measure the experimental affinity data of monoclonal antibody in ascites by means of ELISA and to accurately determine the corresponding affinity constants of monoclonal antibody by amendment of underestimation of affinity constants due to the intrinsic properties of Friguet' s, and shows convenience and reliability.
